Advanced Placement Biology

DNA

The Great Kiwi DNA Extraction and Electrophoresis 

Laboratory

and the

Central Dogma Project

Procedure( Part One )

• Slice kiwi fruit into 6 pieces per student

• Add 20 ml of Suave Clarifying Shampoo.  This Shampoo is essential to the extraction procedure.

• Place in zip lock bag.

• Place bag containing kiwi in another bag

• Use a Falcon tube to mash your Kiwi Fruit.

• Mash for 10 minutes

• Make sure that the Kiwi is almost in a liquid form

• Cool for 2 minutes

• Heat for 2 minutes in the water bath at a temperature of 55^oC

• Spoon mixture onto a cheese cloth covering a beaker

• Use the cheese cloth to strain the lysate or mixture of the Kiwi fruit.

• Pour about 7 ml of lysate into a clean Falcon tube.

• Place on ice for 5 minutes

• Or store overnight

• To extract the DNA Tilt the tube at a 45o angle and drip ice cold 95% ethanol down the sides of the tube until you have a layer of alcohol floating on the top of the lysate .  Take special care not to mix.

• You should see strands of DNA appear at the interface of the alcohol and the lysate.