DNA GEL ELECTROPHORESIS

 

Interpretation of Data

 

Lane 1- Lambda Phage( Hind II)

Lane 2 DNA fragment

Lane 3- Plasmid

Lane 4 Lambda Eco RI 

Lane 5 Kilobase Ladder

 

 

          1             2              3            4                5

 

In order to graph your experimental results, you need to measure the distance that each fragment of DNA traveled in your marker( DNA Standard)  Edvoktek #750

 

1.      In Lane I, Measure the distance from the well for each of the six fragments of DNA.  Check with your reference sheet and complete the chart below.

 

Fragment(Band)

Base Pairs

Distance in mm

1

23,130

 

2

9416

 

3

6557

 

4

4361

 

5

2332

 

6

2027

 

 

2.      Label your graph.  The X axis should be labeled with the linear scale in mm of distance from well

3.      The Y axis should be in bases of DNA.  The Y axis is logarithmic, so that each 1 represents a power of ten

4.      The first one is 100, the second 1 is 1000( 103), the third one is 10,000(104), and the fifth one is 105( 10,000)

5.      Plot the above data on your practice graph

 

Now measure the other lanes of the gel

 

Lane

Fragment 1

Fragment 2

Fragment 3

Fragment 4