Name__________________

Recombinant DNA and Biotechnology( Friday)

  1. Restriction enzymes( restrictiion endonucleases).  What is a restriction enzyme? 

 

 

  1. Explain the significance of a recognition site?

 

 

  1. Explain the principle of DNA electrophoresis.  Use your electrophoresis keywords.

 

Kilobases

Standard( markers )

Migration

Positive electrode and negative electrode.

 

  1. Explain how to cut and splice DNA. 

 

 

  1. How are vectors used to carry new DNA into host cells?

 

 

  1. What is an example of a plasmid vector.

 

 

  1. Explain these terms

 

Polylinker cloning region

 

Ori

Antibiotic resistance gene

 

Gene of interest

 

 

  1. What is a yeast artificial chromosome?

Centromere

Telomere

Ori

Restriction site

Selectable marker

  1. What is the plasmid vector for a plant?

 

 

  1. What is a gene library?

 

 

 

  1. What is a sequencing gel?

 

 

  1. The polymerase chain reaction makes multiple copies of DNA in a test tube.  Explain this process.

 

 

 

 

 

 

 

  1. List some uses of Biotechnology.